The rise of antibiotic resistant infections is an imminent global public health threat, and mitigation measures are required to minimize the risks of transmission and human exposure.
Municipal wastewater treatment plants (WWTPs) are known hotspots for the dissemination of clinically relevant resistant bacteria of human origin to the environment, and simultaneously represent targets for intervention and mitigation strategies. While aerosolized bacteria are found within WWTP, it is largely unknown whether WWTP workers are at risk of elevated resistance carriage. In order to study the occupational and environmental transmission of antibiotic resistance due to human exposure to WWTP-borne bacteria, we will assess carriage of extended-spectrum betalactamase (ESBL) and carbapenemase-producing Enterobacteriaceae and resistance genes in WWTP workers, in residents in the proximity of treatment plants, and in water and air samples – both in countries with low and high antimicrobial resistance (AMR).
Based on microbial cultivation as well as on high-throughput sequencing data and quantitative real-time polymerase chain reaction (qPCR), exposure through ingestion and inhalation will be modelled, and airborne exposure will be derived from geospatial analyses. Further, we will analyse treatment efficiencies of different WWTP processes in terms of AMR reduction, and therewith identify science-based critical control points for interventions.
The focus of this transnational collaboration combining complementary and synergistic European research strengths, is to tackle the increasingly relevant public health threats from antibiotic resistance in WWTP by identifying transmission routes, means of exposure, and proposing risk reduction measures.
- Heike Schmitt, National Institute for Public Health and the Environment (RIVM), Netherlands (Coordinator)
- Carmen Chifiriuc, University of Bucharest, Romania
- Joakim Larsson, University of Gothenburg, Sweden
- Katja Radon, Ludwig-Maximilians-Universität, Germany
- Susan Pettersson, Water and Health, Australia
- Kate Medlicott, World Health Organization (WHO), Switzerland
- Peter Ulleryd, Västra Götaland och Regionala Strama Södra Älvsborgs Sjukhus, Sweden
The rise of antibiotic resistant infections is a global public health threat. Municipal wastewater treatment plants (WWTPs) are known hotspots for the spread of antibiotic resistant bacteria to the environment, and also represent targets for interventions. It is largely unknown whether WWTP workers are at risk of elevated carriage of antibiotic resistant bacteria.
In order to address the transmission of antibiotic resistance in WWTPs, the JPI project AWARE assesses specific resistant bacteria (ESBL and carbapenemase-producing Enterobacteriaceae) as well as antibiotic cresistance genes in WWTP workers, in residents in the proximity of treatment plants, and in water and air samples. These are studied in countries with low and high antimicrobial resistance (AMR). Based on microbial cultivation as well as on molecular microbiological analyses, human exposure is modelled. Treatment efficiencies of different WWTP are determined.
This transnational European collaboration combines complementary and synergistic research strengths, and tackles the public health threats from antibiotic resistance in WWTP by identifying transmission routes, means of exposure, and proposing risk reduction measures.
- AWARE project website
- AWARE project partner Katja Radon profile page (in German)
- Research in RIVM on antibiotic-resistant bacteria in relation to occupation and lifestyle
- PlosOne, 2020. Whole genome sequencing snapshot of multi-drug resistant Klebsiella pneumoniae strains from hospitals and receiving wastewater treatment plants in Southern Romania
- MEDICHUB MEDIA, 2018. Review on methods for analyzing the antibiotic resistance in wastewater samples
- Environment International, 2018. Selective concentration for ciprofloxacin resistance in Escherichia coli grown in complex aquatic bacterial biofilms
- Journal of Environmental Protection, 2017. Physico-Chemical and Microbiological Assessment of Organic Pollution in Plain Salty Lakes from Protected Regions