Yeast-based biosensors for the specific and accessible detection of pathogens and antimicrobial resistance (AntiRYB)
The aim of this project is establishing a method based on yeast biosensors that can detect with high specificity pathogens from different sources to develop a new, fast and specific diagnostic tool for resistant pathogens. We will achieve this by joining together strong research groups on antimicrobial resistance, systems biology, and strain engineering at SINTEF, Chalmers University and National Medicines Institute. Particular focus will be given to the detection of ESBL or carbapenemase-producing strains belonging to the emerging ESKAPE group of resistant pathogens.
The biosensor is developed using the yeast Saccharomyces cerevisiae as host, which will be engineered to express specific receptors able to recognise unique molecules produced by the pathogens. The ligand-receptor binding initiates a cascade mechanism that activates the genes for the production of a red pigment visible to the naked eye. Using the biosensors, we aim to identify molecular markers specific for resistant pathogen strains, to enable fast, easy and inexpensive point-of-use profiling of resistant pathogens.
- Geir Klinkenberg, SINTEF, Norway (Coordinator)
- Verena Siewers, Chalmers University of Technology, Sweden
- Alicja Kuch, National Medicines Institute, Poland