This project has connected many transnational academic resources to investigate the transmission success of the Escherichia coli ST131 clone. E. coli is the most common cause of urinary tract and bloodstream infections worldwide. Resistance to widely used antibiotics for the treating E. coli infections is common and widespread.
Completed project
A single E. coli clone, ST131, is mainly responsible for this global AMR pandemic causing millions of antibiotic-resistant infections annually. It remains unclear which features of ST131 had resulted in the biggest antimicrobial resistance succes of the 2000s.
The ST131TS project created a combined European-Canadian consortium that investigated the transmission dynamics of ST131. The study explored transmission of resistance and virulence genes and how they contributed to the success of ST131. The project also investigated transmission of ST131 among humans, animals and different environments.
The project has showed that ST131 is a public health concern in Canada and Europe and has provided information to better understand the spread and managing infections due to multidrug resistant E. coli. Only by understanding how antibiotic resistance evolves and spreads in bacterial populations, can we begin to overcome such resistance.
ST131 adapts rapidly to environmental changes; we need to know why and how. This project will serve as a model to predict what can possibly happen in the future with the continuing emergence of multidrug resistant clones among bacteria.
Project partners
- Johan Pitout,University of Calgary, Canada (Coordinator)
- Neil Woodford, ARHI-UK, United Kingdom
- Fernando Baquero, Ramón y Cajal Institute for Health Research (IRYCIS), Spain
- Marie-Hélène Nicolas-Chanoine, Beaujon Hospital/ Paris VII University, France
- Laurent Poirel, University of Fribourg, Switzerland
- Alvaro Pascual, Fundación Púbica para la Gestión de la Investigación de Salud en Sevilla, Spain
- Jean-Yves Madec, National Agency for Food, Environmental and Occupational Health & Safety (ANSES), France
- Tarah Lynch, University of Calgary, Canada
Project resources
Tools
- Bioinformatic freetware-AccNET: A comparative genomic tool for accessory genome analysis using bipartite networks.
- PATO: Pangenome Analysis Toolkit
Publications
- Antimicrob Agents Chemother, 2020. Emerging Antimicrobial Resistant High-Risk clones among Klebsiella pneumoniae: ST307 and ST147
- Appl Environ Microbiol, 2020. A Comprehensive Account of Escherichia coli Sequence Type 131 in Wastewater Reveals an Abundance of Fluoroquinolone-Resistant Clade A Strains
- Clinical Microbiol and Infection, 2020. Molecular epidemiology of Escherichia coli causing bloodstream infections in a centralized Canadian region: a population-based surveillance study
- Infection, Genetics and Evolution, 2020. The evolutionary puzzle of Escherichia coli ST131
- Front Microbiol, 2020. Pathogenic Escherichia coli in Dogs Reveals the Predominance of ST372 and the Human-Associated ST73 Extra-Intestinal Lineages
- mBio, 2020. Simulating multi-level dynamics of antimicrobial resistance in a membrane computing model
- Eurosurveillance, 2019. Emergence of diversity in carbapenemase-producing Escherichia coli ST131, England, January 2014 to June 2016
- J Antimicrob Chemother, 2019. Emergence of the C1-M27 cluster in ST131 Escherichia coli from companion animals in France
- medRxiv, 2021. Bacterial genotypic and patient risk factors for adverse outcomes in Escherichia coli bloodstream infections: a prospective molecular-epidemiological study
- Front Microbiol. 2019. Association Between Kinetics of Early Biofilm Formation and Clonal Lineage in Escherichia coli.
- Infect Immun. 2020. Success of Escherichia coli O25b:H4 Sequence Type 131 Clade C Associated with a Decrease in Virulence.
- J Antimicrob Chemother. 2021. Spatial distribution of Escherichia coli ST131 C subclades in a centralized Canadian urban region.
- Emerg Infect Dis. 2020. Trends in Population Dynamics of Escherichia coli Sequence Type 131, Calgary, Alberta, Canada, 2006-2016.
- Infect Genet Evol. 2020. The evolutionary puzzle of Escherichia coli ST131.
- Appl Environ Microbiol. 2020. A Comprehensive Account of Escherichia coli Sequence Type 131 in Wastewater Reveals an Abundance of Fluoroquinolone-Resistant Clade A Strains.
- Clin Microbiol Rev. 2019. Global Extraintestinal Pathogenic Escherichia coli (ExPEC) Lineages.
- Diagn Microbiol Infect Dis. 2018. The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria.
- Antimicrob Agents Chemother. 2021. Antioxidant Molecules as a Source of Mitigation of Antibiotic Resistance Gene Dissemination.
- Antimicrob Resist Infect Control, 2018. Intestinal colonization due to Escherichia coli ST131: risk factors and prevalence.
- J Antimicrob Chemother. 2021. Effect of RecA inactivation on quinolone susceptibility and the evolution of resistance in clinical isolates of Escherichia coli.
- Antimicrob Agents Chemother. 2021. Antioxidant Molecules as a Source of Mitigation of Antibiotic Resistance Gene Dissemination.
- Antimicrob Agents Chemother.2020. Simulating the Influence of Conjugative-Plasmid Kinetic Values on the Multilevel Dynamics of Antimicrobial Resistance in a Membrane Computing Model.
- Nat Microbiol. 2019. Prediction of the intestinal resistome by a three-dimensional structure-based method.
- J Antimicrob Chemother. 2021. Detection in livestock of the human pandemic Escherichia coli ST131 fimH30(R) clone carrying blaCTX-M-27.
- Front Microbiol. 2021. The Origin of Niches and Species in the Bacterial World.
- Nat Microbiol. 2019. Defining and combating antibiotic resistance from One Health and Global Health perspectives.
- Front Microbiol. 2019. Gene Transmission in the One Health Microbiosphere and the Channels of Antimicrobial Resistance.
- Methods Mol Biol. 2020. Statistical Analysis of Accessory Genome.
